ABSTRACT
Objective: To observe the clinical effect and safety of 125I seed implantation combined with chemotherapy in treatment of advanced colorectal cancer with liver metastasis. Methods: Totally 27 cases of advanced colorectal cancer with 60 liver metastasis were enrolled. 125I seed implantation was performed, followed by second-line chemotherapy. The clinical effect, safety and prognosis of the above treatments were observed. Results: Totally 1 995 125I seeds were successfully implanted into 60 liver metastasis([33.41±24.79]seeds in each lesion), and the dose distribution was uniform. Postoperative adverse reactions were mild and recovered after symptomatic treatment. There was no significant difference of liver function before and 1 week after 125I seed implantation. Six patients were treated with oxaliplatin combined regimen and 21 with irinotecan combined regimen 1 week after 125I seed implantation. At the end of 3 and 6 cycles of chemotherapy, the lesion diameter and carcinoembryonic antigen (CEA) level significantly reduced compared with those before implantation (both P< 0.001). The median progression-free survival (PFS) and median overall lifetime (OS) was 8.1 months and 14.6 months, respectively. Conclusion: 125I seed implantation combined with second-line chemotherapy is safe and effective in near future for treating advanced colorectal cancer patients with liver metastasis.
ABSTRACT
Objective: To observe the effect of integrated gastrointestinal nutritional tube loaded with double 125I strands applied in esophageal cancer with grade dysphagia. Methods: Interventional catheter technology was used to open the occlusive segment of esophagus in 10 patients with esophageal cancer and grade dysphagia. Then the new integrated tube was fed along the guide wire with 125I segment needs crossing the esophageal occlusion segment for brachytherapy. The technical success rate and clinical success rate were counted. The dosimetry parameters were recorded and compared before and 3 days after operation, while the therapeutic effect were estimated according to Karnofsky score and Neuhaus dysphagia grading 2 months after operation. The patients were followed up for 6 months to observe the prognosis. Results: All 10 patients underwent successful tube insertion. The technical success rate was 100%, and the clinical success rate was 70%. No serious complication occurred. There was no statistically significant difference of 90% of gross tumor volume (GTV) received dose (D90%), GTV minimum peripheral dose (mPD), GTV received 200% and 100% of the prescribed dose volume percentage (V200%, V100%), conformal index (CI) nor external index (EI) before and after operation (all P>0.05). Karnofsky score and Neuhaus grading were significantly improved 2 months after operation (both P<0.01), and the local tumor control rate was 70%. During 6 months' follow-up, dysphagia reoccurred in 1 case, 2 cases died, while no relapse was found in 7 patients. Conclusion: The integrated gastrointestinal nutritional tube loaded with double 125I strands can achieve gastrointestinal nutrition and brachytherapy at the same time,which is effective and safe for treating esophageal cancer and grade dysphagia.
ABSTRACT
Objective: To observe the effect of 125I on T24 transitional cell carcinoma of nude mouse. Methods: Totally 40 T24 transplanted tumor nude mice were divided into high, medium, low activity and control groups (each n=10), and 125I seeds with activity of 0.9 mCi (33. 3 MBq), 0.6 mCi (22. 2 MBq), 0.3 mCi (11. 1 MBq) and 0 mCi (nuclide free) were implanted in the tumor center, respectively. The 90% target absorbed dose (D90), tumor inhibition rate (IR), radiation reaction grade (RRG) of HE staining, apoptosis index and B-cell lymphoma-2 (Bcl-2) protein expression were analyzed and compared among groups 10 days and 20 days after implantation. Results: D90 and IR of nude mice with high, medium and low activity groups decreased gradually 10 and 20 days after 125I seed implantation (all P<0.05). The necrosis was obvious within 5 mm around the tumor, and the higher the seed activity, the longer the time, the wider the ranges of necrosis. RRG of high activity group 10 and 20 days after 125I seed implantation were higher than that in low activity group and control group (all P<0.05). Meanwhile, the apoptotic index of high, medium and low activity groups gradually decreased, the expression of Bcl-2 protein gradually increased (all P<0.05). Conclusion: 125I seeds can significantly inhibit the growth of T24 metastatic cell carcinoma in nude mouse. Promoting the apoptosis of tumor cells may be one of the mechanisms.
ABSTRACT
Objective To investigate the safety and efficacy of combination of CT-guided multiple minimally invasive techniques in the treatment of lung cancer. Methods All 34 patients with lung cancer (78 lesions) were treated with CT-guided physical ablation (39 lesions), chemical ablation (14 lesions) and/or 125I seed implantation (25 lesions). The efficacy and safety of the treatment were evaluated. Results CT-guided minimally invasive treatments were successfully performed in all 34 patients. During operation, a small amount of hemoptysis occurred in 5 patients, while mild to moderate pain were observed in 4 patients. Pneumothorax occurred in 4 patients after operation. No serious complication,such as massive hemorrhage or bronchial pleural fistula occurred intraoperation nor postoperation. According to the follow-up with CT, there were 69 (69/78, 88.46%) significantly effective lesions, 5 (5/78, 6.41%) stable lesions and 4 (4/78, 5.13%) advanced lesions. Conclusion Combination of CT-guided multiple minimally invasive techniques are safe and effective for treating lung tumor.
ABSTRACT
Objective: To explore the application value of 3D-printing non-coplanar template (3D-PNCT) assisted CT-guided 125I seed implantation through dosimetry analysis. Methods: A total of 15 patients underwent 3D-PNCT assisted CT-guided 125I seed implantation. Posture fixation and set-up were performed with CT-simulator devices. Before operation, pre-plan was carried out, and 3D-PNCT was printed. 125I seeds were implanted into the lesions according to pre-plan after set-up and following postoperative verification. The dosimetric parameters, including doses of pre-plan and post-operation 90%, 100% target volume (D90, D100) and volume percent of 90%, 100%, 150% prescribed doses (V90, V100, V150) were compared. Results: 3D-PNCT assisted CT-guided 125I seed implantation were successfully accomplished in 18 lesions of all 15 patients. The median time of 3D-PNCT set-up was (11.62±2.57)min. Post-operation gross tumor volume (GTV) was more than that in pre-plan ([64.25±50.16]cm3 vs [57.37±44.25]cm3; t=-3.163, P=0.006). The number of implantated seeds were more than that in pre-plan (46.39±24.18 vs 43.78±21.63; t=-2.636, P=0.017). Post-operation V100 and V150 were less than those in pre-plan (both P0.05). Conclusion: 3D-PNCT set-up can be quickly and precisely accomplished using CT-simulator devices. 3D-PNCT assisted CT-guided 125I seed implantation can accurately performed through well match of post-operation main dosimetric parameters with those in pre-plan.
ABSTRACT
Objective: To explore the efficacy of 125I particle bile duct intraluminal and intratumoral implantation for treatment of unresectable pancreatic head carcinoma (PHC). Methods: Clinical data of 72 patients with unresectable PHC were analyzed retrospectively. Among them 37 patients were treated with 125I particle bile duct intraluminal and intratumoral implantation (125I group), and 35 cases underwent laparoscopic palliative surgery (PS group). The postoperative liver function, complications, medial survival time and survival rate at the end of follow-up were compared between the two groups. Results: The levels of serum total bilirubin, alanine aminotransferase and aspartate aminotransferase significantly decreased compared with those of preoperation, the level of serum albumin significantly decreased at 1, 3 and 6 months of postoperation in both groups, especially in 125I group at 3 and 6 months of postoperation (all P<0.05). The incidence of postoperative complications was 28.57% (10/35) in PS group and 21.62% (8/37) in 125I group, and there was no significant difference between the two groups (P=0.496). The medial survival time was 9 months in PS group and 11 months in 125I group (P=0.041). Conclusion: 125I particle bile duct intraluminal and intratumoral implantation can effectively alleviate symptoms of bile duct obstruction and prolong survival time of PHC patients.
ABSTRACT
Objectiv: To observe the efficacy of long-term indwelling of gastrointestinal tube with 125I seeds chains in patients with advanced esophageal cancer. Methods: Long-term indwelling of gastrointestinal tube with 125I seeds chains were performed on 11 patients with advanced esophageal cancer. The success rate of operation was recorded. Eating difficulty classification (Stooler grade), wall thickness of esophageal lesions in the largest layer, effective rate and adverse reactions such as chest pain, bleeding were calculated before and 2 months after operation. Results: The success rate of operation was 100% (11/11). Two months after operation, the 11 patients' eating difficulty improved at different degree, the Stooler grade of dysphagia decreased from preoperative (3.55±0.52)grade to (1.09±0.70)grade. The maximum wall thickness of esophageal lesions decreased from preoperative (21.65±4.50)mm to (11.38±4.20)mm after operation, and the wall thickness contraction rate was (48.89±10.50)%. The effective rate was 90.91% (10/11), without severe complications such as esophageal fistula and hemorrhage. No rupture, loss or displacement of 125I seeds was found on the recovered gastrointestinal tube. Conclusion: Long-term indwelling of gastrointestinal tube with 125I seeds chains is effective in the treatment of advanced esophageal cancer.
ABSTRACT
Objective To investigate the clinical significance of monitoring the serum levels of Thyroglobulin (Tg) and Thyroglobulin Autoantibody (TgAb) in the 131I ablation therapy for patients with differentiated thyroidcarcinoma (DTC).Methods 102 patients with DTC treated by 131I ablation therapy in our hospital from May 2014 to July 2016 were selected.The serum levels of Tg and TgAb were detected by radioimmunoassay before 131I ablation therapy,6 months after I 13 1I ablation therapy and 12 months after 131I ablation therapy.The body imaging were performed at seventh days after 1311 ablation therapy.Results The serum levels of Tg and TgAb at 6 months and 12 months after surgery were significantly lower than those before operation,and the difference were statistically significant (t =9.260,17.507,2.534,4.797,P ＜ 0.05).The serum levels of Tg and TgAb in metastasis group were higher than that in non-metastasis group (t =10.257,7.353,P ＜0.05).The serum levels of Tg and TgAb in the effective group were lower than those in the ineffective group,and the difference were statistically significant (t =7.325,4.978,P ＜ 0.05).The positive rate of Tg combined with TgAb was similar with 131I-WBS screening methods and the difference was not statistically significant (P ＞ 0.05).The positive rate of Tg screening method and the positive rate of TgAb were higher than that of 131I-WBS screening method,but the difference was not statistically significant (P ＞ 0.05).Conclusions The level of serum Tg and TgAb is an important reference index for the efficacy,metastasis and recurrence of DTC after operation,and Tg combined with TgAb has a higher accuracy in the screening prognosis of DTC after operation.
ABSTRACT
Objective To propose a novel preoperative planning method for laparoscopic ultrasound-guided implantation of I125 seeds in treating pancreatic cancer based on CT images,and to evaluate the feasibility of this treatment.Methods CT images of a pancreatic cancer patient were transmitted into the treatment planning system (TPS) developed by our laboratory,to obtain three-dimensional representations of the tumor and surrounding organs.All the positions of puncture were selected with the aid of virtual template,then possible needle paths and the number of necessary I125 seeds were planned,and the radiation dose was evaluated.Results A total of 21 needle paths with 45 seeds implantation into the tumor were planned.In the preoperative planning,the dosage parameters D90 and V100 of the tumor were 12 481 cGy and 92.83％,which was relatively eligible for the clinic requirement,whereas of suurounding non-tumor pancreatic tissue was 0 and 0.40％,respecyively.Conclusion Laparoscopic ultrasound-guided implantation of I125 seeds can be considered as a useful method for treating pancreatic cancer with accurate implantation of 125I seeds and satisfying effect of local control for the tumor in theory.
ABSTRACT
Objective To evaluated the safety and clinical efficacy of CT-guided percutaneous radioactive 125I seeds implantation in treatment of spinal metastatic tumors.Methods Totally 20 cases (23 lesions) of spinal metastatic tumors with spinal compression and severe back pain were treated by CT-guided percutaneous 125I seeds implantation.Intractable pain and nerve function before and after therapy were evaluated.And the postoperative cumulative local tumors control rates and cumulative survival rates were calculated.Results The median follow-up period was 14 months (range 7-32months).There was no patient lost follow-up.And no severe complication occurred.Intractable pain significantly relieved one month after therapy.The neural retention rate of 12 patients with nerve function impairment was 85.00％ (17/20) and the neural function recovery rate was 30.00％ (6/20) three months after 125 I seeds'implantation.The local tumors control rates in 3-,6-,and 12-month were 100％,100％ and 90％,respectively.The survival rates in 6-and 12-month was 100％and 78.81％,respectively.Conclusion CT-guided percutaneous radioactive 125I seeds implantation for spinal metastatic tumors is safe and feasible.It can relief pain and improve neural function effectively.
ABSTRACT
Objective To investigate the incidence of radioactivity uptake in thymus combined with serum thyroglobulin (Tg) increase in differentiated thyroid cancer (DTC) patients after high-dose 131I treatments,in order to discuss the mechanism of thymus iodine uptaking and Tg increasing.Methods Retrospective analysis of the laboratory examinations and 131Iwhole body scan (131I-WBS) images in 316 DTC patients were performed.The radioactivity uptake in thymus and the Tg level were observed.Results Among 316 patients (total 735 case-times),4 patients of 5 cases-times 131I-WBS showed radioactivity uptake in thymus,accounting for 0.68％ (5/735).All the radioactivity uptake in thymus were found by posttreatment 131I whole body scan (Rx-WBS) and after the second radioactive iodine treatment.For 1 of 4 patients,Rx-WBS still showed thymic uptake 131I after the third radioactive iodine treatment.The serum Tg increased in 3 patients (4 caestimes Rx-WBS) of radioactivity uptake in thymus with the Tg level before Rx-WBS was 13.80 μg/L,＞300.00 μg/L,16.40 μg/L,20.60μg/L,respectively.Conclusion In order to avoid the inappropriate administration of radioiodine therapy,thymic uptake should be identified carefully in DTC patients whose radioactivity uptake is only found at the upper mediastinal and combined with serum Tg increase.
ABSTRACT
Objective To investigate the efficacy and influential factors of 131I treatment for lung metastases from DTC.Methods Fifty patients (18 males,32 females;age (40.8±13.2) years) with lung metastases from DTC who underwent 131I treatment from October 2007 to December 2012 were retrospectively analyzed.The efficacy of 131 I treatment was assessed using 131I imaging and determination of serum Tg level after 6 months.The possible factors affecting efficacy included patients' age,gender,operation method,pathological classification,the diagnostic time of pulmonary metastasis,serum Tg level at diagnosis,131I uptake pattern,characteristics of other imaging modalities,cervical lymph node metastases and extrapulmonary distant metastases (assign 1 for metastases,0 for no metastases).Univariate and multivariate analyses (Student t test,Fisher exact test and logistic regression) were performed to investigate the factors.Results The rates of complete remission,partial response and invalid of 131I treatment were 20％ (10/50),74％ (37/50) and 26％ (13/50) respectively.Univariate analysis showed that age(t =2.019,P＜0.05),gender (P =0.032),serum Tg level at diagnosis (t =2.646,P＜ 0.05),findings of other imaging modalities (P =0.039),and extrapulmonary distant metastases(P=0.023) were the factors influencing outcome of 131I treatment.Multivariate logistic regression analysis showed that the influential factors included age,serum Tg levels and extrapulmonary distant metastases.The regression equation was as follows:logit P =2.127-0.056× age-0.163×Tg level-1.280×extrapulmonary distant metastasis (x2=10.484,P＜0.001).Aged patients,a significant increase of Tg level and extrapulmonary distant metastases indicated a poor prognosis.Conclusions 131I treatment is an effective method for lung metastases from DTC.The patients with younger age,lower Tg levels,no other distant metastases had good response to 131I treatment.
ABSTRACT
Objective To discuss differentiated thyroid carcinoma (DTC)postoperative lymph node metas-tasis and clinical use and the effect of 131 I treatment.Methods to185 patients with lymph node metastasis of DTC were selected by random sampling method.The standard 131 I treatment was administrated,ultrasound,CT and Tg chan-ges and imaging characteristics of 131 I treatment were evaluated the clinical curative effect was determined.Results Imaging changes:the whole group of patients,153 patients (82.7%)were effective.153 patients with effective treat-ment,105 cases examined LMDTC completely eliminate (56.8%),48 cases examination revealed narrowing (25.9%).Mere lymph node metastasis after treatment was better than that of patients with viscera metastasis,imaging examination results were statistically significant differences (χ2 =2.57,P =0.02).After treatment,imaging examina-tion result in patients with smaller lymph node was superior to that in the patients with relatively large diameter lymph nodes (P >0.05).Tg level changes:there was a significant reduction in the Tg 97 cases of 185 patients (51.1%), and general decline in 54 patients (29.2%).Tg improvement effect of patients with mere lymphatic metastasis after treatment was better than other viscera metastasis in patients with statistically significant differences (χ2 =3.41,P =0.01).Lymph node Tg improved after treatment in patients with small diameter,which was better than that with other viscera metastasis patients with statistically significant differences(χ2 =6.34,P =0.02).Conclusion Comprehensive curative effect of 131 I treatment of lymph node metastasis postoperatively in patients with DTC is distinct,and is worth popularizing in clinical use of DTC.
ABSTRACT
Objective To study the effect of network peer education on the 131I treatment adherence in patients with thyroid cancer.Methods A total of 120 patients with thyroid cancer who performing 131 I treatment from April 2012 to April 2014.They were divided into intervention group 61 cases and control group 59 cases according to the hospital ward number.Control group was given routine health education and intervention group was given network peer education.The data from the habits,drug therapy,grasp the situation,the equivalent monitoring of the relevant knowledge of 4 different dimensions of treatment adherence for patients were investigated and compared before discharge by using questionnaire and examination method.Results The incidence of the good habits,drug therapy,grasp the situation,and the equivalent monitoring reaching the standard were 96.7％(59/61),98.4％(60/61),96.7％(59/61),100.0％(61/61) in intervention group,and 54.2％(32/59),76.3％(45/59),79.7％(47/59),89.8％(53/59) in control group,there were significant differences,x2=29.54,13.38,8.47,4.56,P＜0.01 or ＜0.05.Conclusion Network peer education for patients with thyroid cancer during the 131I treatment can effectively improve the patient's treatment adherence,has positive significance for the treatment.
ABSTRACT
Objective To explore the clinical efficacy of image-guided 125I radioactive seed interstitial implantation therapy for unresectable pancreatic cancer.Methods 25 patients with unresectable pancreatic cancer evaluated by retrospective follow-up were enrolled in this study,13 patients received radioactive seeds implantation while 12 patients were given non-surgical treatment.We observe and compare the clinical benefits,objective curative effect,complications,adverse reaction,survival between the two groups of patients.Results Compared with the non-surgical treatment group,the clinical benefit rate in the radiotherapy seed implantation group was 92％ (12/13) while that of the non-surgical group was 42％ (5/12),the difference was of statistically significance.The numbers of cases evaluated as effective were 6 (46％) and 4 (33 ％) respectively,the difference was not statistical significant (x2 =0.427,P ＞ 0.05);The radioactive seed implantation group had no serious postoperative complications;3 cases who received subsequent chemotherapy in radioactive seed implantation group(23％,3/13) and 3 cases in non-surgical treatment group(25％,3/12)suffered from serious adverse reactions,the difference was of no statistical significance(x2 =0.013,P ＞0.05);Comparing the survival rate between the two groups,x2 =0.001,P =0.969,the difference was of no statistical significance.Conclusions The therapy of 125I radioactive seed implantation for unresectable pancreatic cancer significantly relieves cancer caused pain and improves quality of life.
ABSTRACT
Objective To study the changes of the serum levels of thyroid peroxidase antibody (TPOAb) and thyroglobulin antibody (TGAb) after 131I treatment in patients with Graves disease,and investigate the correlation between the early hypothyroidism and serum levels of TPOAb,TGAb after 131I treatment.Methods The serum levels of TPOAb and TGAb of 167 patients with Graves disease were detected before treatment and 3,6,12,18 months after 131I treatment.According to the levels of TPOAb and TGAb,all patients were divided into positive antibodies group (72 cases) and negative antibodies group(95 cases),and the incidence of the early hypothyroidism was compared between two groups after 131I treatment.According to the incidence of the early hypothyroidism,all patients were divided into early hypothyroidism group(64 cases) and non-early hypothyroidism group(103 cases),and the variations of thyroid function and thyroid autoantibody were detected.Results The incidence of hypothyroidism in 18 months after 131I treatment in positive antibodies group was 19.4％(14/72),while that in negative antibodies group was 7.4％(7/95),and there was significant difference between two groups (P ＜ 0.05).The positive rate of TPOAb and TGAb was highest in 6 months after 131I treatment,but there was no significant difference compared with that before treatment (P ＞ 0.05).The positive rate of TPOAb and TGAb was decreased in 3,12,18 months after 131I treatment.There was significant difference in the positive rate of TPOAb and TGAb between the time before treatment and 18 months after 131I treatment[15.6％(26/167),19.8％(33/167)](P＜ 0.01).The serum level of TPOAb and TGAb in early hypothyroidism group was higher than that in non-early hypothyroidism group [(433.2 ± 98.4) kU/L vs.(211.4 ± 76.5) kU/L; (165.7 ± 41.6) kU/L vs.(89.5 ± 21.1) kU/L,P ＜0.01].Conclusions The levels of TPOAb and TGAb before treatment is related to the probability of the early hypothyroidism,and play a guided role in 131I treatment of Graves disease.
ABSTRACT
Objective To investigate the effect of Iodine 125 seeds short time low dose rate irradiation on perineural invasion (PNI) in pancreatic cancer Capan-2 cells,and explore its molecular mechanism.Methods The co-culture model was established by co-culturing the dorsal root ganglion (DRG) of SD rat and Capzn-2 cells line,while Capan-2 culture model and DRG culture model was also established.Iodine 125 seeds short time low dose rate irradiation tablet was used for the 3 models,and the model without irradiation was used as control.Cancer cell and DRG growth was observed under inverted microscopy,surface of neurite and cell colony growth was determined by image analysis software.The concentration of nerve growth factor (NGF),transforming growth factor-α (TGF-α) in cell culture supernatant and matrigel solution was tested by ELISA,and the expression of neurotrophin-3 (NT-3) mRNA was detected by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR).Results In the co-culture model,neurite of DRG showed a direction to cancer cells and had a concentrated growth towards cancer cells.And Capan-2 cells formed more colonies towards neurite.However,in irradiation groups,the symbiotic phenomenon was inhibited to some degree.Increased surface of neurite in co-culture model at 5th day was 290.15 ± 12.08,which was significantly higher than that in DRG group (124.83 ± 6.96,P ＜ 0.01),but the surface of neurite was decreased to 201.53 ± 12.20 after irradiation (P ＜0.01).Increased surface of Capan-2 cell was 300.47 ± 12.99,which was significantly higher than that in Capan-2 group (199.30 ± 8.60,P ＜ 0.01),but the surface of Capan-2 was decreased to 202.35 ± 7.97 after irradiation (P ＜ 0.01).NT-3 mRNA was seldom or not expressed in supernatant of co-culture model,but it was strongly expressed (0.68 ± 0.04) after irradiation (P ＜ 0.05).The concentration of NGF and TGF-α in supernatant of co-culture model were (27.56 ± 13.73),(40.86 ± 20.73) ng/ml,after irradiation they were increased to (94.98 ± 33.80),(157.54 ± 83.76) ng,/ml,and the difference between the two groups was statistically significant (P＜0.05 or ＜0.01).The concentration of NGF and TGF-α in matrigel lysate of co-culture model were (60.42 ± 33.03),(64.39 ± 21.52)ng/ml,after irradiation they were increased to (132.52 ±53.01),(138.38 ±83.58)ng/ml,and the difference of NGF concentration between the two groups was statistically significant (P ＜ 0.05).Conclusions Iodine-125 seeds short-time low-dose rate irradiation could inhibit interactions between nerve and Capan-2 cells,and the mechanism may be related to up-regulation of cancer cells perineural invasion promoter NGF,TGF-α and NT-3.
ABSTRACT
Objective To probe into optimal delivered dose of 131I therapy in hyperthyroidism and influencing factors.Methods Data of 6374 patients with hyperthyroidism receiving 131I therapy were summarized and analyzed.The patients were differentiated into Graves group (GD) and multinodular toxic goiter group (NGD) after 131I therapy.To compare incidence of hypothyroidism between two groups after 131I therapy,partial correlations analysis was performed between delivered dose and other variables influencing factors among cured patients.Results After 131I therapy incidence of permanently hypothyroidismin patients with Graves' group was much higher than that in patients with multinodular toxic goiter group(17.01％,0.39％,Pearson x2 value =704.8,P ＜ 0.01).Delivered dose of 131I therapy related closely to thyroid size,with or without nodules and thyroid rigidity(r =0.469,0.484,0.553).Disposable cured rate of 131I therapy was higher than 63.99％.Conclusion 131I therapy is optimal to multinodular toxic goiter.Delivered dose of 131I therapy will be increased for patients with bigger and stiff mass for therapeutic effects.
ABSTRACT
PURPOSE: Current study is focused on extraction with methanol, purification, labeling with 131I using iodogen method of the yarrow plant and investigating in vivo biological activity using biodistribution and imaging studies on healthy animal models. The aim of the study is to contribute plant extracts to discover new drugs in the diagnosis and treatment of several diseases. METHODS: Nine female and nine male healthy Wistar albino rats, which were approximately 100-150 g in weight, were used for biodistribution studies. For imaging studies four healthy male Balb-C mice were used. Quality control studies were done utilizing thin layer radio chromatography (TLRC) and high performance liquid chromatography (HPLC) methods. For biodistribution studies, 131I radiolabeled Peak 7 (131I-Peak 7) was sterilized and injected into the tail veil of rats and imaging studies were obtained using Kodak FX PRO in vivo Imaging System. RESULTS: The radiolabeling yield of each purified the bioactive extracts of the yarrow plant, seven peaks was between 79 and 92%. The highest radiolabeling yield was calculated for 131I radiolabeled seventh peak (131I-Peak 7) (92.78±5.04, n=5). For this reason the biodistribution and imaging studies were done for 131I-Peak 7. That's why; these studies with Peak 7 were carried out. CONCLUSION: Peak 7 was radiolabeled with 131I in high yield for using imaging and therapeutic studies in nuclear medical applications.
OBJETIVO: O atual estudo tem por objetivo a extração com metanol, purificação, marcação com I131 usando o método direto de marcação da planta Achillea, para investigar in vivo a atividade biológica usando biodistribuição e estudos de imagem em modelos animais saudáveis. O objetivo do estudo é contribuir com extratos de plantas para descobrir novas drogas para o diagnóstico e tratamento de várias doenças. MÉTODOS: Nove fêmeas e nove machos ratos Wistar albino saudáveis, com aproximadamente 100 a 150g de peso foram usados para estudos de biodistribuição. Para estudos de imagem, quatro camundongos Balb-C machos e saudáveis foram usados. Estudos de controle de qualidade foram realizados usando métodos de cromatografia de camada fina e cromatografia líquida de alta performance. Para estudos de biodistribuição, pico 5 radiografado com I131 (I131-Peak 7) foi esterilizado e injetado na veia da cauda dos ratos e estudos de imagem foram obtidos usando Sistema de Imagem Kodak FX PRO in vivo. RESULTADOS: O retorno radiomarcado de cada extrato bioativo purificado da planta Achillea sete picos estavam entre 79 e 92%. O retorno com maior marcação foi calculado para I131 sétimo pico (I131-Peak 7) (92,78±5,04, n=5). Por esta razão os estudos de biodistribuição e de imagem foram feitos para I131-Peak 7. CONCLUSÃO: Peak 7 foi radiomarcado com I131 em alto retorno para uso em estudos terapêuticos e de imagens nas aplicações médicas nucleares.
Subject(s)
Animals , Female , Mice , Rats , Achillea/chemistry , Iodine Radioisotopes/chemistry , Isotope Labeling/methods , Plant Extracts/isolation & purification , Chromatography, High Pressure Liquid , Disease Models, Animal , Methanol , Mice, Inbred BALB C , Plant Extracts/pharmacology , Rats, WistarABSTRACT
Objective To analyze the clinical effects of internal radiation of iodine-125 seed implantation in the treatment of advanced pancreatic carcinoma.Methods 22 patients with unresectable and advanced pancreatic carcinoma were treated by the internal and interstitial radiation using iodine-125 seed implantation.Results Survival of 20 months was observed in 5 patients,10 ～ 20 months in 9 patients,2 ～10 months in 8,patients with an average of (13.47 ± 8.12) months.Among the 22 patients,complete response was obtained in 5 patients,partial response in 8 patients,no response in 7 paticnts,and the other showing PD.The response rate( CR + PR) was 59％.Conclusions Intra - operative internal radiation by iodine - 125 seed implantation does show some therapeutic effects for advanced pancreatic carcinoma.